Product information thermo scientific dreamtaq dna. Thermo scientific f530l phusion highfidelity dna polymerase is supplied with 5x phusion hf buffer, 5x phusion gc buffer, dmso and 50 mm mgcl2 solution. Due to a proprietary mutation in the so called dutp binding pocket of phusion, phusion. Phusion is a registered trademark and property of thermo fisher scientific. Thermo scientific phusion highfidelity dnapolymerase. Robust reactions maximal success with minimal optimization. New phusion green format is a combination of phusion dna polymerase and 5x phusion green buffers. Phusion highfidelity dna polymerase from finnzymes oy. It ensures higher sensitivity, longer pcr products and higher yields compared to conventional taq dna polymerase. Phusion highfidelity dna polymerase 2 ul thermo fisher. Thermo scientific phusion hot start ii high fidelity dna polymerase is the most accurate hot start dna polymerase on the market.
Thermo scientific phusion highfidelity pcr kit fisher. Thermo scientific phusion highfidelity dna polymerase offers both high. Thermo scientific pfu dna polymerase is a highly thermostable dna polymerase from the hyperthermophilic archaeum pyrococcus furiosus. Thermo scientific phusion highfidelity dna polymerase. Thermo scientific phusion u hot start pcr master mix. Manufactured and qualitycontrolled at new england biolabs, thermo scientific phusion highfidelity dna polymerase offers both high fidelity and robust performance, and thus can be used for all pcr applications. New england biolabs uk ltd phusion highfidelity pcr kit. Hot start highfidelity dna polymerase thermo scientific taq however, before adding the overhangs it is very important to remove all phusion hot start dna polymerase by purifying the pcr product carefully. Incorporating an exciting new technology, phusion dna polymerase brings together a novel pyrococcuslike enzyme with a processivityenhancing domain. Phusion highfidelity pcr kit thermo fisher scientific. Since their introduction, thermo scientific phusion highfidelity dna polymerases have been referenced in thousands of publications for highperformance pcr and have become the choice for a. Q5 q5 hot start highfidelity dna polymerase neb pu pfuultra ii fusion hs dna polymerase agilent pt platinum taq dna polymerase high fidelity invitrogenlife pf phusion hot start flex dna polymerase neb ap accuprime pfx dna polymerase invitrogenlife 4,960 bp 370 bp 450 bp 410 bp 520 bp amplification of a variety of.
Phusion hot start flex dna polymerase is supplied with standard 5x phusion hf buffer, as well as 5x phusion. Thermo scientific phusion dnapolymerase met hoge getrouwheid 500u. Dreamtaq dna polymerase uses the same reaction setup and cycling conditions as conventional taq dna polymerase. To narrow down your search, select desired filters or enter keywords in the. Due to a proprietary mutation in the so called dutp binding pocket of phusion, phusion u overcomes an important limitation of proofreading enzymes it is able to incorporate dutp and read through uracil present in dna templates. This product is manufactured by new england biolabs, inc. Phusion highfidelity dna polymerase exploits this dramatic increase in processivity, resulting in shorter extension times, more robust and high yield amplification, and the ability to. With an error rate 50fold lower than that of taq dna polymerase and 6fold. Product information thermo scientific dreamtaq dna polymerase. Phusion dna polymerase was developed by finnzymes oy, now a part of thermo fisher scientific.
Manufactured and qualitycontrolled at new england biolabs, thermo scientific phusion highfidelity dna polymerase offers both high fidelity and robust performance. The enzyme catalyzes the templatedependent polymerization of nucleotides into duplex dna in the 5 3 direction. The following guidelines are provided to ensure successful pcr using phusion dna polymerase. Phusion hot start ii high fidelity dna polymerase is the most accurate hot start dna polymerase on the market. Thermo scientific phusion highfidelity pcr master mix. Jun 24, 2019 since their introduction, thermo scientific phusion highfidelity dna polymerases have been referenced in thousands of publications for high performance. The processivity of phusion dna polymerase is 10fold greater than that pyrococcus furiosus dna polymerase and twice that of thermus aquaticus dna polymerase. It is licensed and optimized for use in the polymerase chain reaction pcr process.
Phusion dna polymerases from thermo fisher scientific description one of the highest referenced polymerase lines in thousands of publications, the thermo scientific phusion highfidelity polymerases are highly accurate and robust products that provide high yields with short reaction times and enhanced specificity. In addition, phusion dna polymerases also produce higher yields with lower enzyme amounts than other dna polymerases. Phusion is a dna polymerase isolated from pyrococcus. Featuring an error rate 50fold lower than that of taq, and 6fold lower than that of pfu, phusion high. Thermo scientific phusion hot start ii dna polymerase 2 ul optimize pcr with one of the most accurate hot start dna polymerases on the market, featuring extreme specificity and improved performance. Since their introduction, thermo scientific phusion highfidelity dna polymerases have been referenced in thousands of publications for high performance. Phusion dna polymerases from thermo fisher scientific description one of the highest referenced polymerase lines in thousands of publications, the thermo scientific phusion highfidelity.
First isolated from the bacterial thermophile thermus aquaticus, the taq polymerase has become the staple enzyme in molecular biology. Phusion hot start flex dna polymerase is available as standalone enzyme or in a master mix format and enables high specificity amplification of a broad range of templates. High fidelity52x more accurate than taq, 6x more accurate than pfu. The dna template amount is sufficient for performing 20 control reactions in 50 l volume or 50 control reactions in 20 l volume. Thermo scientific phusion highfidelity dna polymerase 2 ul 500u. The enzyme, originally developed by finnzymes, is a fusion of a proofreading polymerase. Product information pfu dna polymerase, recombinant pfu. Affibody inhibits the dna polymerase activity at room temperature and thus prevents the amplification of nonspecific products. Highfidelity dna polymerases are important for applications in which the dna sequence needs to be correct after amplification. Thermo scientific phusion highfidelity dna polymerase promotion get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable dna polymerases.
Phusion dna polymerases thermo fisher scientific us. Taq polymerase from thermo fisher scientific a wide variety of enzymes are available for molecular and biochemical applications. The addition of an aptamerbased inhibitor allows room temperature reaction setup. Thermo scientific f530s phusion highfidelity dna polymerase is supplied with 5x phusion hf buffer, 5x phusion gc buffer, dmso and 50 mm mgcl2 solution. Thermo scientific phusion hot start ii dna polymerase 2 u. Thermo scientific phusion highfidelity dna polymerase promotion. Phusion dna polymerases have become the first choice in highperformance pcr for. Both phusion hf buffer and phusion gc buffer provide 1. Thermo scientific phusion green hot start ii highfidelity.
Phusion dna polymerases from thermo fisher scientific. Difference between phusion and taq polymerase compare the. An ultrapure recombinant thermostable taq dna polymerase obtained by high level expression of the taq dna polymerase gene in e. For the researcher, that translates to easeofuse and convenience. Due to the unique nature of phusion dna polymerases, please pay special. Amplification of whole human mitochondrial dna with thermo pia kuusisto, thermo fisher scientific, vantaa, finland 1 x gc buffer 1 u enzyme total volume 50. Thermo scientific phusion u dna polymerase is a proofreading enzyme 25x taq with a proprietary mutation to read through uracils and incorporate dutp. Thermo scientific phusion highfidelity dna polymerase offers extreme performance for all major pcr applications. The phusion highfidelity dna polymerase brings together a pyrococcuslike enzyme with a processivityenhancing domain. Dna polymerases with high fidelity are important for applications in which the dna sequence needs to be correct after amplification. Phusion is a dna polymerase isolated from pyrococcus furiosus and is mainly used in cloning experiments to increase the fidelity taq dna polymerase is the standard dna polymerase used in the polymerase chain reaction pcr, and it is isolated from the thermostable bacterium, thermus aquaticus. The phusion dna polymerase generates long templates with an accuracy and.
Phusion highfidelity dna polymerase, new england biolabs. The thermo scientific phusion high fidelity dna polymerase offers high performance for all major pcr applications. Thermo scientific phusion u green hot start dna polymerase. Featuring an error rate 50fold lower than that of taq. England biolabs, thermo scientific phusion highfidelity dna polymerase. Its unique structure, a novel pyrococcuslike enzyme fused with a processivityenhancing domain, increases fidelity and speed. It combines the phusion dna polymerase and a reversibly bound, specific. Thermo scientific taq dna polymerase with kcl buffer. Phusion highfidelity dna polymerase high performance for. Pcr protocol for phusion highfidelity dna polymerase m0530 protocols. Phusion highfidelity dna polymerase high performance for all. Finnzymes phusion highfidelity dna polymerase offers extreme performance for all pcr applications. Since their introduction, thermo scientific phusion highfidelity dna polymerases have been referenced in thousands of publications for highperformance pcr and have become the choice for a multitude of demanding applications ranging from reconstruction, design and massivelyparallel, highthroughput sequencing of whole genomes.
Thermo scientific phusion hot start ii dna polymerase 2 ul. Phusion hot start flex dna polymerase offers robust, high fidelity performance and room temperature reaction setup. L the cycling conditions for phusion highfidelity dna that of taq polymerase. Thermo scientific phusion hot start ii highfidelity dna. Its unique structure, a novel pyrococcuslike enzyme. Thermo scientific phusion highfidelity dna polymerase 2 ul. The annealing rules are different from many common dna polymerases such as taq dna polymerases. Phusion highfidelity dna polymerase thermo fisher scientific. Ab0192kcl 1 thermo scientific taq dna polymerase with kcl buffer description. Commercial producers have sinced cloned and expressed the enzyme in e. Taq polymerase from thermo fisher scientific biocompare. Thermo scientific phusion green highfidelity dna polymerase.
Benefits of invitrogen platinum ii taq hotstart dna polymerase. Choose phusion polymerase from thermo for highly accurate, fast and robust pcr. Manufactured and qualitycontrolled at new england biolabs, thermo scientific phusion highfidelity. Thermo scientific dreamtaq dna polymerase is an enhanced taq dna polymerase optimized for all standard pcr applications. The thermo scientific phusion highfidelity dna polymerase offers high performance for all major pcr applications. Phusion dna polymerase combines extreme fidelity with unparalleled speed and robustness a random set of.
Phusion dna polymerases thermo fisher scientific fr. Thermo scientific phusion highfidelity dna polymerases set a gold standard for high performance pcr. The phusion high fidelity dna polymerase brings together a pyrococcuslike. Thermo scientific phusion hot start ii highfidelity dna polymerase combines the phusion dna polymerase and a reversibly bound, specific affibody ligand. Thermo scientific phusion green hot start ii highfidelity dna polymerase 2 ul phusion green hot start ii highfidelity dna polymerase. Thermo scientific phusion highfidelity pcr kit contains all the necessary reagents for pcr including control lambda dna template and primers for 1. Phusion highfidelity dna polymerase new england biolabs. Recombinant taq is a 94kda enzyme with an optimal 53 polymerase activity between 7580 c in the presence of a magnesium cofactor. Fisher scientific taq polymerase now sold as thermo scientific part of thermo fisher scienti. Thermo scientific phusion highfidelity dna polymerase thermo scientific phusion hot start ii highfidelity dna polymerase thermo scientific phusion u hot start dna polymerase thermo scientific phusion flash highfidelity pcr master mix no no no no yes yes yes yes 52x 52x 25x 25x no no yes no blunt blunt blunt blunt. Thermo scientific phusion highfidelity dna polymerase 2 ul 100u. Due to the unique nature of phusion dna polymerases, please pay special attention to the guidelines listed below. Phusion and taq polymerase are two commercially produced thermostable polymerase enzymes that are used in special molecular applications. Phusion highfidelity dna polymerase for high speed and high fidelity pcr, 52x.
Other phusion hot start flex dna polymerase product introduction for high speed and high performance pcr. Difference between phusion and taq polymerase compare. Incubate purified pcr product with 1x taq buffer, 2. Thermo scientific f530s phusion dna polymerase met hoge precisie, wordt geleverd met 5x phusion hfbuffer, 5x phusion gcbuffer, dmso en 50 mm mgcl2oplossing. It combines the phusion dna polymerase and a reversibly bound, specific affibody ligand. The purchase price of this product includes a limited, non. Thermo scientific phusion u dna polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
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